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Cancer Primary Screening

DNA Methylation Detection Kit for Human SDC2, SEPTIN9 and TFPI2 Gene -- COLC (Colorectal Cancer)

Clinical Application

    Colorectal cancer (CRC) affects millions of people worldwide. It is unique in that it progresses slowly, making it 
    preventable, and mortality can be significantly reduced through early detection. Testing using stool samples is more appropriate for screening efforts than colonoscopy because it is easier to conduct the program. Simultaneous testing with FIT not only greatly improves sensitivity and specificity, but is also more acceptable for widespread screening, or home sampling.
     
    DNA Methylation can be a novel marker of prognosis for tumor therapy, which is also a reversible process in which inactivated tumor suppressor genes are expressed through demethylation to exert a tumor suppressor effect. Therefore, demethylation can be used as a new direction in cancer treatment to monitor the dynamics of the disease.

    Intended Use

    This kit is a real-time PCR assay for qualitative detection of DNA methylation in gene SDC2, SEPTIN9 and TFPI2 Gene in human genomic DNA extracted from stool sample.

    General Specification

    Methodology: Real-Time PCR, PCR-Fluorescence Probing

    Sample Type: Stool
    Analytical Sensitivity: 93.63%
    Analytical Specificity: 90.82%
    Precision: CV <5%
    LoD: 1% of  SDC2, SEPTIN9 and TFPI2 methylation in the background of 5 ng human genomic DNA

    Packing Specifications

    24 tests/kit (non-single test/tube)48 tests/kit (non-single test/tube)
     

    Work Flow



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